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Early pathology of lymphoma developing in newborn Syrian hamsters inoculated with human leukemic lymphoblasts
Author(s) -
Liknaitzky David G.,
Takakusu Akira,
Adams Richard A.
Publication year - 1969
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(196901)23:1<94::aid-cncr2820230110>3.0.co;2-2
Subject(s) - parenchyma , pathology , spleen , lymphoblast , connective tissue , medicine , pancreas , peritoneum , lymphoma , lymphatic system , biology , cell culture , immunology , endocrinology , genetics
The fate of cultured tritium‐labeled human leukemic lymphoblasts (CCRF‐CEM cells) has been studied following their intraperitoneal injection into newborn Syrian hamsters. Within the first 24 hr, tumorigenic doses (1.0 × 10 8 ) of cells were implanted into the loose retroperitoneal connective tissue adjacent to the kidney, aorta, and para‐aortic lymphatics. Cells were also seen on the peritoneum, in the connective tissue of the pancreas, and on and invading the splenic capsule. Heavily labeled single cells and small clumps of cells were also seen within the splenic parenchyma at this time. Nontumorigenic inocula (1.0 × 10 7 ) of the lymphoblastic cells behaved essentially like tumorigenic inocula in the first 24 hr, except for the apparent absence of heavily labeled cells within the spleen. Beyond 3 days postimplantation, dilution of the label resulted in less confident identification of individual cells lying outside major tumor aggregations. These observations suggest that early involvement of the splenic parenchyma, possibly by the hematogenous route, may be a requisite for progressive growth of the tumor cell inoculum.