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Potentiating effect of vitamin a on 9‐10 dimethyl 1‐2 benzanthracene—carcinogenesis in the hamster cheek pouch
Author(s) -
Levij I. S.,
Polliack A.
Publication year - 1968
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(196808)22:2<300::aid-cncr2820220207>3.0.co;2-2
Subject(s) - dmba , cheek pouch , carcinogen , acanthosis , benzanthracene , medicine , hamster , vitamin , carcinogenesis , endocrinology , pathology , cancer research , chemistry , cancer , biochemistry , hyperkeratosis
Hamster cheek pouches were painted three times weekly for 2 to 12 weeks with 0.5% 9‐10 dimethyl 1‐2 benzanthracene (DMBA) and/or 10% vitamin A dissolved in liquid paraffin. With vitamin A alone, foci of acanthosis first appeared after 4 weeks. After DMBA alone for 6 weeks intraepithelial carcinoma was present. However, after only 4 weeks application of the combination of DMBA and vitamin A, intraepithelial carcinomas were found and after 6 weeks infiltrating carcinomas were present. After 8 weeks infiltrating carcinomas were also present in pouches treated with DMBA alone but at this stage, as well as after 10 and 12 weeks, the combination of DMBA and vitamin A had induced more anaplastic and larger tumors than DMBA alone. These findings may be the result of an addition of the epithelial hyperplasia effected by vitamin A and the carcinogenic effect of DMBA. Alternatively, the increased permeability of cellular and subcellular membranes induced by vitamin A may facilitate the entry of DMBA into the cells, thus potentiating its carcinogenic effect.