Premium
Bio‐assay of the effects of smoking on dna content of human respiratory epithelium
Author(s) -
Roth Daniel,
Fredrickson Donald T.,
Oppenheim Abraham
Publication year - 1968
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(196806)21:6<1132::aid-cncr2820210614>3.0.co;2-b
Subject(s) - dna , medicine , acriflavine , carcinogenesis , epithelium , carcinogen , respiratory system , physiology , pathology , cancer , microbiology and biotechnology , genetics , biology
A survey of alterations in the DNA content of human oropharyngeal epithelium related to smoking was carried out. The technique used had been developed for mass screening of the DNA content of populations of intact cells and was based on the binding of acriflavine by cells in proportion to their DNA content. Groups of nonsmokers, heavy smokers and cancer cases served to establish the normal and abnormal dye‐binding ranges. Among 14 previously heavy smokers who abstained completely, there was a decline to the normal range in 6 months. The implications of this finding are discussed in connection with carcinogenesis and the application of the technique as a bio‐assay in humans for evaluating cigarettes claimed to be safe is suggested.