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Cytologic studies of glucose‐6‐phosphate dehydrogenase in malignancy
Author(s) -
Cohen Harvey J.,
Elizalde Adolfo,
Miller Sherwood P.
Publication year - 1968
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(196806)21:6<1055::aid-cncr2820210605>3.0.co;2-1
Subject(s) - enzyme assay , medicine , malignancy , dehydrogenase , cytology , pathology , enzyme , lactate dehydrogenase , carcinoma , cancer , ovary , biopsy , biology , biochemistry
A histochemical technique for the determination of glucose‐6‐phosphate dehydrogenase (G6PD) was combined with cytologic studies of malignant tumor cells obtained by aspiration biopsy and touch preparations. Consistently high levels of G6PD activity were found in carcinoma cells of the colon. Lung cancer cells showed no or very weak enzyme activity in most cases. Wide variations in the level of this enzyme activity were noted in carcinoma of the breast and ovary while moderately strong activity occurred in cells from hypernephroma. Lympho‐proliferative diseases and soft tissue sarcomas also showed little or no activity. When combined with other cytohistologic enzyme determinations, patterns of enzyme activity corresponding to particular tissue origins became apparent.