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Tritiated thymidine uptake by ehrlich ascites cells exposed to ethyl heptyloxyacetate
Author(s) -
Basrur Parvathi K.,
Swierenga Sabine H.,
Townsend Gordon F.
Publication year - 1968
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(196805)21:5<888::aid-cncr2820210512>3.0.co;2-y
Subject(s) - thymidine , ascites , mitosis , dna synthesis , medicine , andrology , microbiology and biotechnology , endocrinology , biochemistry , biology , dna
An investigation on the pattern of DNA synthesis was conducted on treated and untreated Ehrlich ascites tumor cells in order to determine the nature of mitotic block induced by ethyl heptyloxyacetate. Ascites cells, aspirated from treated and control mice at 2, 4, 6, 24 and 48 hours after the administration of ethyl heptyloxyacetate, were incubated with tritiated thymidine for 20 min at 37°C and autoradiographic analyses were carried out. The percentage of labeled cells and the average grain count did not vary appreciably in the controls during the experimental period; however, the percentage of labeled cells was the lowest at 4 hours and the grain count approximately 2.5 times that of control at 48 hours in ethyl heptyloxyacetate treated samples. The marked drop in S phase cells in the treated samples at 4 hours is considered to be due to the destruction of cells in mitosis at the time of treatment. The absence of mitotic stages in samples taken before 48 hours and the increased grain count at 48 hours in treated ascites cells seem to be attributable to a delay of G2 phase by ethyl heptyloxyacetate.