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Host defenses in leukemia and Burkitt's tumor
Author(s) -
Klein George,
Klein Eva,
Clifford Peter
Publication year - 1968
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(196804)21:4<587::aid-cncr2820210407>3.0.co;2-2
Subject(s) - burkitt's lymphoma , biopsy , immunofluorescence , cell culture , lymphocyte , medicine , raji cell , immunology , leukemia , lymphoma , antibody , virology , pathology , biology , genetics
Indirect membrane immunofluorescence reaction, obtained by exposure of viable Burkitt lymphoma biopsy cells to the sera of certain Burkitt patients, was studied, particularly in one highly positive serum donor in a state of chemotherapeutically induced regression and free of all signs of disease 32 months after initiation of therapy. His sera gave highly positive reactions against Burkitt biopsy cells from 13 donors while the cells of three donors reacted more weakly. Tests on non‐Burkitt cells from Burkitt or non‐Burkitt donors were negative. These data, together with various tests for isoantibodies, indicate that the reactivity against the Burkitt target cells is not due to isoantibody. Established Burkitt tissue culture lines of the strains Jijoye, B35M, EB3 and SL1 gave strong reactions with Mutua's serum; Kudi, Ogun and Raji strains were completely negative. The activity of Mutua's serum resided in its IgG fraction. Eight other established tissueculture lines of non‐Burkitt origin also were negative. All lines tested reacted equadlly well with isoantibodies present in the serum of a multiply transfused patient. Three “Burkitt” sera of non‐African origin gave preliminary reaction patterns against three Burkitt and three non‐Burkitt lines that resembled the pattern obtained with African sera. Admixture of 6000 R x‐irradiated cells of the reactive Jijoye line to cells from various nonreactive lines resulted in the transfer of membrane reactivity in a number of cases. No such transfer occurred when irradiated Burkitt biopsy cells or three other reactive Burkitt tissue‐culture lines were used as donors.