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Biosynthesis of steroid hormones in a human feminizing adrenal carcinoma
Author(s) -
Bryson Melvin J.,
Young Richard B.,
Reynolds William A.,
Sweat Max L.
Publication year - 1968
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(196803)21:3<501::aid-cncr2820210321>3.0.co;2-e
Subject(s) - androstenedione , pregnenolone , estrone , hydroxylation , testosterone (patch) , endocrinology , medicine , steroid , hormone , hydroxyprogesterone , adrenocortical carcinoma , chemistry , adrenal gland , biology , biochemistry , enzyme , androgen
Pregnenolone‐4‐ 14 C, progesterone‐4‐ 14 C, 17α‐hydroxyprogesterone‐4‐ 14 C, 11‐deox‐ycorticosterone‐4‐ 14 C, androstenedione‐4‐ 14 C and testosterone‐4‐ 14 C were incubated with a homogenate of a feminizing human adrenocortical carcinoma. Estrone, 17β‐estradiol were isolated and identified in the phenolic fraction from substrates: pregnenolone, progresterone, androstenedione and testosterone. From androstenedione 19‐hydroxyandrostenedione and 19‐nortestosterone were identified as metabolic products. Hydroxylation reactions were active for carbons 6, 17 and 21 and appeared to proceed at rates normal to adrenal gland tissue; 16‐hydroxylation was not observed. In the tissue homogenates 11β‐hydroxylation was not observed; however, it could be demonstrated when supported with the addition of components from bovine adrenocortical mitochondria, thus indicating the presence of only part of the 11β‐hydroxylating system.