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In vitro neoplastic transformation of hamster pineal cells by three oncogenic DNA viruses
Author(s) -
Orme S. Kirby,
Wells Samuel A.,
Rabson Alan S.,
Wurtman Richard J.
Publication year - 1968
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(196803)21:3<477::aid-cncr2820210319>3.0.co;2-6
Subject(s) - hamster , in vitro , virology , virus , biology , transformation (genetics) , malignant transformation , simian , dna , cell culture , mesocricetus , oncovirus , microbiology and biotechnology , polyoma virus , cancer research , gene , genetics
Explant cultures of 3 to 4‐week‐old hamster pineal glands were infected with four oncogenic DNA viruses: polyoma virus, human adenovirus 12, simian virus 40 (SV40) and the LLE 46 strain of adenovirus 7 (the adenovirus 7‐SV40 “hybrid”). The tissues infected by polyoma, SV40 and LLE 46 underwent transformation. Three histologically distinct tumors were produced by subcutaneous injections of these transformed cells into adult irradiated hamsters. Although the tumors differed histologically, hydroxyindole‐O‐methyl transferase (HIOMT), an enzyme found exclusively in the pineal gland, was present in all tumors produced by the injected cells and in the cultures of the transformed cells.