Premium
Effect of urethane, hydroxyurethane and hydroxyurea on synthesis of nucleic acid
Author(s) -
Perpich Joseph,
Yarbro John W.,
Kennedy B. J.
Publication year - 1968
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(196803)21:3<456::aid-cncr2820210316>3.0.co;2-k
Subject(s) - in vivo , dna synthesis , nucleic acid , in vitro , dna , rna , pharmacology , biochemistry , ehrlich ascites , biology , medicine , microbiology and biotechnology , tumor cells , cancer research , genetics , gene
The effect of urethane, hydroxyurethane and hydroxyurea on synthesis of nucleic aicd in 6C3HED mouse ascites tumor was measured by incorporation of radiophorus. Hydroxyurethane and hydroxyurea inhibited synthesis of DNA both in vivo and in vitro with little or no inhibition of RNA synthesis except at very high doses. In vitro this inhibition was seen as early as 30 min after addition of the drug. These drugs had an equal capacity to inhibit DNA synthesis in equimolar concentrations. Urethane produced no inhibition of DNA or RNA synthesis in vitro or invivo during the early time periods and at 4 hours in vivo only slight depression of DNA synthesis was noted.