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Histologic changes in bronchial tubes of cigarette‐smoking dogs
Author(s) -
Auerbach Oscar,
Hammond E. Cuyler,
Kirman David,
Garfinkel Lawrence,
Stout Arthur Purdy
Publication year - 1967
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(196712)20:12<2055::aid-cncr2820201202>3.0.co;2-m
Subject(s) - medicine , beagle , morning , bronchopneumonia , surgery , pathology
Tracheostomy was performed on ten beagles (nine males and one female) aged 9 to 30 months. The tracheostomy was kept open by a Teflon tube. The dogs smoked morning and afternoon of each day (7 days a week). The number of cigarettes smoked per day was gradually increased to a maximum of 12 per day. Five dogs died during the experiment. Four of these had pulmonary infarction and one died of bronchopneumonia. The remaining five dogs were sacrificed after 421 days of smoking. Ten male Beagle dogs were kept as controls; two of these had tracheostomies kept open by Teflon tube. The tracheobronchial tree was dissected from the lungs and divided into portions (133) similar to those taken from humans. A microscopic slide was made of each portion. Altogether, 2508 histologic sections were obtained from the 20 dogs. The slides were put in random order and were identified only by a serial number. No atypical nuclei were found in any of the nonsmoking dogs and none in the dog that had smoked 24 days. A few were found in every section from the other nine smoking dogs. The proportion of cells with atypical nuclei was considerably greater in five dogs that were sacrificed after 421 days than those that had died on the 229 and 278 days respectively. Three dogs showed lesions which were classified as dyskeratosis.