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Effect of radium treatment on activity and distribution of some enzymes in cancers of the human cervix uteri
Author(s) -
Goldberg David M.,
Ayre Heather A.,
Pitts Janet F.
Publication year - 1967
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(196709)20:9<1388::aid-cncr2820200904>3.0.co;2-6
Subject(s) - radium , cervix , enzyme , medicine , ribonuclease , specific activity , microsome , endocrinology , biochemistry , biology , chemistry , rna , cancer , gene , radiochemistry
The activities and distribution of nucleases, adenosine deaminase and dehydrogenases of lactate (LDH), isocitrate and phosphogluconate were examined in tissue biopsies obtained from 16 patients with carcinoma of the cervix uteri before and after therapeutic radium treatment and fractionated into mitochondrial, microsomal and supernantant preparations. A significant increase in the specific activities of alkaline and acid ribonuclease occurred in all three cytoplasmic fractions after radium treatment and a significant fall in LDH specific activity took place in the supernantant fraction. The specific activities of the other enzymes were unaffected or moderately reduced. Radiation did not alter the protein content of the mitochondrial and microsomal fractions relative to tissue weight but the protein content of the supernant was halved, leading to significant reduction in the activities of several enzymes relative to tissue weight. This work has revealed early biochemical changes induced by radium treatment with potential application to radiotherapeutic assessment.