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Continuous culture of seven new cell lines (SK‐L1 to 7) from patients with acute leukemia
Author(s) -
Clarkson Bayard,
Strife Annabel,
de Harven Etienne
Publication year - 1967
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(196706)20:6<926::aid-cncr2820200603>3.0.co;2-4
Subject(s) - acute myelomonocytic leukemia , acute myeloblastic leukemia , leukemia , ploidy , medicine , cell culture , lymphoblastic leukemia , doubling time , acute leukemia , immunology , cytogenetics , in vitro , cancer research , biology , chromosome , genetics , gene
Continuous suspension cultures of seven lines of cells derived from the blood of seven patients with acute leukemia who had large numbers of circulating leukemic cells are described. Three cultures have been growing for more than 18 months and the other four for more than six months. Two of the cultures were obtained from children with lymphoblastic leukemia and five from patients with myeloblastic or myelomonocytic leukemia. In one of the myelomonocytic cultures, after several weeks in vitro , some of the blasts originally present underwent partial differentiation into pseudoeosinophils; these later died out, to be replaced by continuously‐growing blasts. In all of the established cultures, the cells resembled primitive blast forms morphologically, were highly motile, had diploid or near‐diploid modal numbers of chromosomes, and had doubling times of between 20 and 70 hours.