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Leukemogenic effect of human leukemic materials. Attempts to enhance effect in mice by combination of materials with inactivated mouse leukemia virus
Author(s) -
Sinkovics Joseph G.,
Shullenberger C. C.,
Howe Clifton D.,
Bertin Barbara A.
Publication year - 1967
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(1967)20:5<846::aid-cncr2820200543>3.0.co;2-d
Subject(s) - leukemia , virus , virology , bone marrow , spleen , murine leukemia virus , biology , medicine , immunology
Human leukemic bone marrow cells or fluids from cultures of such cells were mixed with heat‐inactivated (56°C for one hour) Rauscher mouse leukemia virus and the mixtures were inoculated into two to three‐day‐old Swiss mice of a low‐leukemia line. Control mice were inoculated with (1) the human material mixed with heat‐inactivated normal mouse spleen extract and (2) the heatinactivated Rauscher virus mixed with fluids from normal human cell cultures. Sixteen human leukemic materials were tested with equivocal results except in one instance. When mixed with heat‐inactivated Rauscher virus, tissue culture fluids from lymph node and bone marrow cells of a 15‐year‐old girl with acute leukemia repeatedly caused leukemia with significantly higher incidence than the corresponding controls. Theoretically, an increased leukemogenic potency of human leukemic materials and inactivated murine leukemia virus can be explained by recombination of human and murine leukemia viruses (“genome rescue”) but other mechanisms, such as phenotypic mixing, multiplicity reactivation of the murine leukemia virus or increased activity of virus‐decoating enzymes, are also possible.