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Certain properties of leukocytic cathepsins in health and disease
Author(s) -
FraenkelConrat J.,
Chew W. B.,
Pitlick F.,
Barber S.
Publication year - 1966
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(196610)19:10<1393::aid-cncr2820191010>3.0.co;2-h
Subject(s) - leukemia , polycythemia vera , hemoglobin , medicine , chronic myelogenous leukemia , cathepsin , immunology , biochemistry , chemistry , enzyme
The effect of pH on the catheptic activity of leukocytes from normal, polycythemic and leukemic (lymphatic, myelogenous, eosinophilic) blood has been investigated. When acid‐denatured hemoglobin was used as the substrate, the leukocytic cathepsins from polycythemia and myelogenous and eosinophilic leukemia were more active than normal. All showed optima at pH 3.5 except polycythemia, which was pH 4.0. When urea‐alkali‐denatured hemoglobin was used as substrate, the leukocytic cathepsins of polycythemia were more active than normal; of myelogenous leukemia, slightly less than normal; of eosinophilic leukemia, even less than normal; of lymphatic leukemia, exceedingly less than normal. All optima were around pH 8.5. When lymphocytes and granulocytes were separated from normal blood, the lymphocytes showed more activity than the granulocytes at pH 3.5 and less than granulocytes at pH 8.5. These observations indicate that the differences noted in the catheptic activities of cells as isolated from various leukemias are not due to a preponderance of one type of cell but rather to inherent deranged chemical properties of the cells.