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Comparative histochemical studies of l‐leucyl‐β‐naphthylamidase in ovarian epithelial tumors
Author(s) -
Long Margaret E.,
Hosannah Yvonne T.
Publication year - 1966
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(196607)19:7<909::aid-cncr2820190704>3.0.co;2-a
Subject(s) - serous fluid , ovarian carcinoma , stroma , pathology , carcinoma , epithelium , ovary , proteolytic enzymes , enzyme , immunohistochemistry , biology , medicine , ovarian cancer , cancer , biochemistry
Abstract The histochemical characterization of activity of the multi‐proteolytic enzyme system, the L‐leucyl‐β‐naphthylamidases, is compared in ovarian papillary serous tumors, their metastases, their cells in effusions and their cells grown in tissue culture. Two histochemical aspects of L‐NA‐ase activity are reported: (1) Sites of activity are newly recorded with constancy in the carcinoma cells and stroma of the ovarian carcinoma in all phases of its life history; (2) Analysis of levels of activity, expressed as a function of time of appearance of reaction products during incubation, reveal 2 categories of activity—low in benign and differentiated tumors, high in more malignant neoplasms while still higher in metastatic lesions than their primaries. Ovarian carcinoma cells grown in tissue culture and in effusions demonstrate high activity. The possibility that epithelial cells and stroma contain different enzymes is suggested.