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Biochemical studies of human tumors. I. Estimation of tumor malignancy from metabolic measurements in vitro
Author(s) -
Bickis I. J.,
Henderson I. W. D.
Publication year - 1966
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(196601)19:1<89::aid-cncr2820190110>3.0.co;2-e
Subject(s) - malignancy , anaerobic glycolysis , glycolysis , medicine , anaerobic exercise , warburg effect , pathology , in vitro , nucleic acid , metabolism , biochemistry , biology , physiology
Slices from fresh human tumor specimens were incubated in Warburg manometric vessels and the respiration, glycolysis and radioactive tracer incorporation into protein and nucleic acids measured. The sum of 3 relative metabolic parameters—per cent Crabtree effect, per cent aerobic glycolysis and per cent anaerobic efficiency to synthesize protein—was designated as indicator of individual tumor malignancy. In a group of 36 human tumors this metabolic “malignancy index” varied from 110 to 265, which correlated well with histopathological criteria and the clinical course for each patient. In several cases the metabolic malignancy indicators predicted the course of disease better than histopathological criteria.