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A new fold in the scorpion toxin family, associated with an activity on a ryanodine‐sensitive calcium channel
Author(s) -
Mosbah Amor,
Kharrat Riadh,
Fajloun Ziad,
Renisio JeanGuillaume,
Blanc Eric,
Sabatier JeanMarc,
Ayeb Mohamed El,
Darbon Herve
Publication year - 2000
Publication title -
proteins: structure, function, and bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.699
H-Index - 191
eISSN - 1097-0134
pISSN - 0887-3585
DOI - 10.1002/1097-0134(20000815)40:3<436::aid-prot90>3.0.co;2-9
Subject(s) - ryanodine receptor , antiparallel (mathematics) , chemistry , calcium channel , scorpion toxin , stereochemistry , dihydropyridine , biophysics , receptor , biochemistry , calcium , venom , biology , scorpion , physics , organic chemistry , quantum mechanics , magnetic field
We determined the structure in solution by 1 H two‐dimensional NMR of Maurocalcine from the venom of Scorpio maurus . This toxin has been demonstrated to be a potent effector of ryanodyne‐sensitive calcium channel from skeletal muscles. This is the first description of a scorpion toxin which folds following the Inhibitor Cystine Knot fold (ICK) already described for numerous toxic and inhibitory peptides, as well as for various protease inhibitors. Its three dimensional structure consists of a compact disulfide‐bonded core from which emerge loops and the N‐terminus. A double‐stranded antiparallel β‐sheet comprises residues 20–23 and 30–33. A third extended strand (residues 9–11) is perpendicular to the β‐sheet. Maurocalcine structure mimics the activating segment of the dihydropyridine receptor II‐III loop and is therefore potentially useful for dihydropyridine receptor/ryanodine receptor interaction studies. Proteins 2000;40:436–442. © 2000 Wiley‐Liss, Inc.