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UV cured biocompatible membrane for biosensor application
Author(s) -
Ng LooTeck,
Guthrie James T,
Yuan Yong J
Publication year - 2000
Publication title -
polymer international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.592
H-Index - 105
eISSN - 1097-0126
pISSN - 0959-8103
DOI - 10.1002/1097-0126(200009)49:9<1017::aid-pi557>3.0.co;2-t
Subject(s) - chitosan , fourier transform infrared spectroscopy , materials science , differential scanning calorimetry , immobilized enzyme , biosensor , methacrylate , chemical engineering , polymer chemistry , polymer , atom transfer radical polymerization , polymerization , chemistry , organic chemistry , nanotechnology , composite material , thermodynamics , enzyme , physics , engineering
As a natural product, chitosan has the inherent properties of being biocompatible, biodegradable, and non‐toxic. These properties make chitosan an ideal candidate for inclusion in matrices designed for use in enzyme immobilization for clinical analysis. The current work demonstrates the feasibility of using chitosan in electrochemical biosensor fabrication. The enzyme, sulfite oxidase (SOD), was covalently immobilized onto the matrix of chitosan–poly(hydroxyethyl methacrylate) (chitosan–pHEMA), a natural/synthetic polymer hybrid material obtainable via a UV curing process. An electron transfer mediator, p ‐benzoquinone was coupled onto the polymer network for the activation of chitosan–pHEMA copolymer, after completion of the photo‐induced polymerization reaction. Chitosan–pHEMA was characterized using Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC) techniques. The biological activity of the immobilized SOD and the electroactivity of the coupled p ‐benzoquinone were examined. © 2000 Society of Chemical Industry