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Subtelomeric sequence from the right arm of Schizosaccharomyces pombe chromosome I contains seven permease genes
Author(s) -
Hunt Cherryl,
Moore Karen,
Xiang Zheng,
Hurst Suzanne M.,
McDougall Rachel C.,
Rajandream MaireAdèle,
Barrell Barclay G.,
Gwilliam Rhian,
Wood Valerie,
Lyne Michael H.,
Aves Stephen J.
Publication year - 2001
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/1097-0061(20010315)18:4<355::aid-yea676>3.0.co;2-p
Subject(s) - biology , permease , genetics , peptide sequence , orfs , schizosaccharomyces pombe , gene , nucleic acid sequence , open reading frame , saccharomyces cerevisiae , transporter
The sequence has been determined of 80 888 bp of contiguous subtelomeric DNA, including the isp5 gene, from the right arm of chromosome I of Schizosaccharomyces pombe ; 27 open reading frames (ORFs) longer than 100 codons are present, giving a density of one gene per 3.0 kb. Seven of the predicted proteins are members of the major facilitator superfamily (MFS) of transport proteins, including four amino acid permease homologues, bringing this family of amino acid permease sequences to 17 in Sz. pombe , and a phylogenetic analysis is presented. Also encoded is an allantoate permease homologue, a sulphate permease homologue and a probable urea active transporter. Predicted non‐membrane proteins include a 1‐aminocyclopropane‐1‐carboxylate deaminase (ACC deaminase), a class III aminotransferase, serine acetyltransferase, protein‐ L ‐isoaspartate O ‐methyltransferase, α‐glucosidase, α‐galactosidase, esterase/lipase, oxidoreductase of the short‐chain dehydrogenase/reductase (SDR) family, aldehyde dehydrogenase, formamidase, amidase, flavohaemoprotein, a putative translation initiation inhibitor and a protein with similarity to a filamentous fungal conidiation‐specific protein. The remaining six ORFs are likely to encode proteins, either because they have sequence similarity with hypothetical proteins or because they are known to be transcribed. Introns are scarce in the sequenced region: only three ORFs contain introns, with only one having multiple introns. The sequenced region also contains a single Tf1 transposon long terminal repeat (LTR). The sequence is derived from cosmid clones c869, c922 and c1039 and has been submitted to the EMBL database under entries SPAC869 (Accession No. AL132779), SPAC922 (AL133522) and SPAC1039 (AL133521). Copyright © 2001 John Wiley & Sons, Ltd.