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Hyperthermotolerant fission yeast mutations, sow1 and sow2 , suppress the cell cycle defect and stress sensitivity of MAP kinase kinase wis1 Δ
Author(s) -
Prochnik Simon,
Fantes Peter
Publication year - 2001
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/1097-0061(200102)18:3<229::aid-yea658>3.0.co;2-r
Subject(s) - schizosaccharomyces pombe , biology , mitosis , cell cycle , microbiology and biotechnology , yeast , cell division , mutant , kinase , mitogen activated protein kinase , protein kinase a , genetics , cell , saccharomyces cerevisiae , gene
Wis1 is a mitogen‐activated protein kinase kinase (MAPKK) that regulates mitosis and mediates stress responses in the fission yeast, Schizosaccharomyces pombe . wis1 Δ strains are viable but stress‐sensitive and show a mitotic delay. At high temperatures, wis1 Δ cells cease division but cellular growth continues. Mutations that suppress the heat sensitivity of a wis1 Δ strain were isolated and map to two apparently novel loci, sow1 (for s uppressor o f w is1 Δ) and sow2 . In addition to suppressing wis1 Δ heat sensitivity, sow1 and sow2 can suppress wis1 Δ osmosensitivity and cell cycle defects. sow1 and sow2 mutants in a wis1 + background were able to grow at higher temperatures than wild‐type and sow1 showed a mitotic advance. The sow genes may therefore define a novel connection between stress tolerance and cell cycle control. Copyright © 2000 John Wiley & Sons, Ltd.