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The cloning and characterization of the CDC50 gene family in Saccharomyces cerevisiae
Author(s) -
Radji Maksum,
Kim JongMyong,
Togan Takahiro,
Yoshikawa Hiroshi,
Shirahige Katsuhiko
Publication year - 2001
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/1097-0061(200102)18:3<195::aid-yea660>3.0.co;2-l
Subject(s) - biology , gene , mutant , genetics , saccharomyces cerevisiae , gene product , missense mutation , mutation , null allele , phenotype , microbiology and biotechnology , gene expression
We have cloned a gene that complements the cold‐sensitive growth of cdc50‐1 mutant strain of Saccharomyces cerevisiae at 14°C. The CDC50 gene was found to be identical to YCR094w on chromosome III and contains 1173 nucleotides encoding 391 amino acids. We found a missense mutation at the first initiation codon of cdc50‐1 . The disruption of the CDC50 gene revealed that it is not essential for growth, but the disruptant caused the same cold‐sensitive phenotype as cdc50‐1 , suggesting that the cdc50‐1 is a null mutation resulted from the mutation in the first codon. The cdc50‐1 mutant arrests at START in G 1 phase at the non‐permissive temperature. The CDC50 gene product has strong structural similarity to two other proteins in Saccharomyces cerevisiae encoded by YNR048w and YNL323w . The over‐expression of either YNR048w or YNL323w suppressed the cdc50‐1 mutant and the double disruption of either CDC50 and YNR048w or CDC50 and YNL323w resulted in a severe slow‐growth phenotype. We conclude that these three genes constitute a family with redundant function. We also found that the CDC39 gene was a multicopy suppressor of cdc50‐1 mutation, suggesting that the CDC50 family is involved in regulation of transcription via CDC39 . Copyright © 2000 John Wiley & Sons, Ltd.