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The Schizosaccharomyces pombe GPI8 gene complements a Saccharomyces cerevisiae GPI8 anchoring mutant
Author(s) -
ShamsEldin Hosam,
Azzouz Nahid,
Eckert Volker,
Blaschke Thomas,
Kedees Mamdouh H.,
Hübel Andreas,
Schwarz Ralph T.
Publication year - 2001
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/1097-0061(200101)18:1<33::aid-yea648>3.0.co;2-z
Subject(s) - biology , schizosaccharomyces pombe , mutant , anchoring , gene , saccharomyces cerevisiae , yeast , homologous chromosome , amino acid , genetics , microbiology and biotechnology , structural engineering , engineering
The final step in glycosylphosphatidylinositol (GPI) anchoring of cell surface proteins consists of a transamidation reaction, in which preassembled GPI donors are substituted for C‐terminal signal sequences in nascent polypeptides. The Saccharomyces cerevisiae GPI8 gene ( ScGPI8 ) encodes a protein which is involved in the GPI transamidation reaction. We have cloned and isolated the Schizosaccharomyces pombe GPI8 homologous gene ( SpGPI8 ). The SpGPI8 gene encodes a protein of 411 amino acids with a calculated molecular weight of about 47 kDa. It shows 53.5% identity with the ScGPI8 and complements a S. cerevisiae GPI8 anchoring mutant. The nucleotide sequence data reported in this paper appears in the EMBL Data Bank nucleotide sequence database with Accession No. AJ250428. Copyright © 2000 John Wiley & Sons, Ltd.