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Tca5, a Ty5‐like retrotransposon from Candida albicans
Author(s) -
Plant Ewan P.,
Goodwin Timothy J. D.,
Poulter Russell T. M.
Publication year - 2000
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/1097-0061(200012)16:16<1509::aid-yea638>3.0.co;2-r
Subject(s) - biology , retrotransposon , genetics , genbank , long terminal repeat , primer binding site , reverse transcriptase , primer (cosmetics) , transfer rna , integrase , nucleic acid sequence , transposable element , gene , genome , rna , chemistry , organic chemistry
This report describes the identification and characterization of a retrotransposon, termed Tca5, from the pathogenic yeast Candida albicans . Tca5 has identical 685 bp LTRs flanking 4218 bp of internal sequence within which lies a single long ORF. Immediately internal to the left LTR is a primer binding site complementary to an internal portion of the initiator methionine tRNA and upstream of the right LTR is a polypurine tract. The ORF predicts a protein containing all the conserved motifs characteristic of Gag, protease, integrase, reverse transcriptase and RNaseH. Genomic Southern blots probed with Tca5 sequences show that it is a low copy number element and is present at different loci in different strains. This, together with the apparently intact structure of Tca5, suggests that it has transposed very recently. Potentially full‐length Tca5 transcripts were detected in some strains raising the possibility that some copies of Tca5 may still be active. Phylogenetic analyses and other sequence comparisons suggest that Tca5 is most closely related to the Ty5 element of Saccharomyces cerevisiae and S. paradoxus . The nucleotide sequence of Tca5 has been submitted to GenBank under Accession No. AF093417. Copyright © 2000 John Wiley & Sons, Ltd.