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Analysis of 114 kb of DNA sequence from fission yeast chromosome 2 immediately centromere‐distal to his5
Author(s) -
Xiang Zheng,
Moore Karen,
Wood Valerie,
Rajandream MaireAdèle,
Barrell Barclay G.,
Skelton Jason,
Churcher Carol M.,
Lyne Michael H.,
Devlin Karen,
Gwilliam Rhian,
Rutherford Kim M.,
Aves Stephen J.
Publication year - 2000
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/1097-0061(200011)16:15<1405::aid-yea625>3.0.co;2-h
Subject(s) - biology , microbiology and biotechnology , genetics , schizosaccharomyces pombe , gene , saccharomyces cerevisiae
One hundred and fourteen kilobase pairs (kb) of contiguous genomic sequence have been determined immediately distal to the his5 genetic marker located about 0.9 Mb from the centromere on the long arm of Schizosaccharomyces pombe chromosome 2. The sequence is contained in overlapping cosmid clones c16H5, c12D12, c24C6 and c19G7, of which 20 kb are identical to previously reported sequence from clone c21H7. The remaining 93 781 bp of sequence contains 10 known genes ( cdc14 , cdm1 , cps1 , gpa1 , msh2 , pck2 , rip1 , rps30‐2 , sad1 and ubl1 ), 32 open reading frames (ORFs) capable of coding for proteins of at least 100 amino acid residues in length, one 5S rRNA gene, one tRNA Pro gene, one lone Tf1‐type long terminal repeat (LTR) and one lone Tf2‐type LTR. There is a density of one protein‐coding gene per 2.2 kb and 22 of the 42 ORFs (52%) incorporate one or more introns. Twenty‐one of the novel ORFs show sequence similarities which suggest functions of their products, including a cyclin C, a MADS box transcription factor, mad2‐like protein, telomere binding protein, topoisomerase II‐associated protein, ATP‐dependent DEAH box RNA helicase, G10 protein, ubiquitin‐activating e1‐like enzyme, nucleoporin, prolyl‐tRNA synthetase, peptidylprolyl isomerase, δ‐1‐pyrroline‐5‐carboxylate dehydrogenase, protein transport protein, coatomer epsilon, TCP‐1 chaperonin, β‐subunit of 6‐phosphofructokinase, aminodeoxychorismate lyase, a phosphate transport protein and a thioredoxin. The sequence has been submitted to the EMBL database under Accession Nos AL021839, AL022104, AL031786 and AL035085. Copyright © 2000 John Wiley & Sons, Ltd.