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Progression of cell cycle monitored by dielectric spectroscopy and flow‐cytometric analysis of DNA content
Author(s) -
Asami Koji,
Takahashi Keiko,
Shirahige Katsuhiko
Publication year - 2000
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/1097-0061(200011)16:15<1359::aid-yea631>3.0.co;2-e
Subject(s) - cell cycle , biology , cell sorting , dna , cell division , cell culture , cell , dielectric , interphase , flow cytometry , phase (matter) , cell growth , microbiology and biotechnology , analytical chemistry (journal) , biophysics , genetics , materials science , chromatography , chemistry , optoelectronics , organic chemistry
A dielectric method has already been developed for the real‐time monitoring of cell cycle progression in synchronized cell culture (Asami et al ., 1999). This method, in combination with DNA content analysis by fluorescence‐activated cell sorting (FACS), was applied to the synchronized cell culture of a CDC28‐13th mutant ( Saccharomyces cerevisiae ). In synchronous cell growth, relative permittivity ε (or dielectric constant) for the culture broth showed cyclic changes at low frequencies below 0.5 MHz, being correlated to phases in the cell cycle that were simultaneously determined by FACS. The ε increased in the period from S phase to G 2 phase and decreased between M and G 1 phases. Peaks in these cyclic changes of ε indicated the time when daughter cells segregated from mother cells. Copyright © 2000 John Wiley & Sons, Ltd.