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Simultaneous overexpression of enzymes of the lower part of glycolysis can enhance the fermentative capacity of Saccharomyces cerevisiae
Author(s) -
Peter Smits Hans,
Hauf Jörg,
Müller Susanne,
Hobley Timothy J.,
Zimmermann Friedrich K.,
HahnHägerdal Bärbel,
Nielsen Jens,
Olsson Lisbeth
Publication year - 2000
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/1097-0061(200010)16:14<1325::aid-yea627>3.0.co;2-e
Subject(s) - pyruvate decarboxylase , biology , biochemistry , recombinant dna , phosphoglycerate mutase , alcohol dehydrogenase , phosphoglycerate kinase , strain (injury) , pyruvate kinase , chemostat , dehydrogenase , enzyme , saccharomyces cerevisiae , glycolysis , yeast , bacteria , gene , genetics , anatomy
Recombinant S. cerevisiae strains, with elevated levels of the enzymes of lower glycolysis (glyceraldehyde‐3‐phosphate dehydrogenase, phosphoglycerate mutase, phosphoglycerate kinase, enolase, pyruvate kinase, pyruvate decarboxylase and alcohol dehydrogenase) were physiologically characterized. During growth on glucose the enzyme levels in the recombinant strains (YHM4 and YHM7) were 1.1–3.4‐fold higher than in the host strain (CEN.PK.K45). The recombinant strains were grown in aerobic or anaerobic batch cultures on glucose or a mixture of glucose and galactose. The specific ethanol production rates in the recombinant strains were the same as for the host strain and the physiological behaviour of the recombinant strains and the host strain was similar. When the cellular demand for ATP was increased by means of glucose pulses (final concentrations of 3.9 g/l or 2.0 g/l, respectively) to aerobic chemostat cultures maintained at a dilution rate of 0.08/h, the specific carbon dioxide production rate (qCO 2 ) of CEN.PK.K45 accelerated at 6×10 −3 mmol/g/min 2 during the first 15 min, whereas during the same time period the qCO 2 of YHM7 accelerated twice as fast at 12×10 −3 mmol/g/min 2 , indicating a higher fermentative capacity in the recombinant strain. Copyright © 2000 John Wiley & Sons, Ltd.