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A network of proteins around Rvs167p and Rvs161p, two proteins related to the yeast actin cytoskeleton
Author(s) -
Bon Elisabeth,
RecordonNavarro Patricia,
Durrens Pascal,
Iwase Masayuki,
Tohe Akio,
Aigle Michel
Publication year - 2000
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/1097-0061(20000930)16:13<1229::aid-yea618>3.0.co;2-q
Subject(s) - orfs , biology , yeast , saccharomyces cerevisiae , actin , actin cytoskeleton , cytoskeleton , microbiology and biotechnology , gene , genetics , open reading frame , peptide sequence , cell
The Rvs161p and Rvs167p proteins of Saccharomyces cerevisiae , homologues of higher eukaryotes' amphiphysins, associate with actin and appear to be involved in several functions related to the actin cytoskeleton. In order to identify partners of the Rvsp proteins, yeast libraries constructed in two‐hybrid vectors were screened using either Rvs167p or Rvs161p as a bait. The selected candidates, representing 34 ORFs, were then tested against both Rvsp proteins, as well as domains of Rvs167p or Rvs161p. Among the most significant ones, 24 ORFs were specific preys of Rvs167p only and two gave interactions with Rvs161p only. Interestingly, five ORFs were preys of both Rvs161p and Rvs167p (RVS167, LAS17, YNL094w, YMR192w and YPL249c). Analysis of putative functions of the candidates confirm involvement of the Rvsp in endocytosis/vesicle traffic, but also opens possible new fields, such as nuclear functions. Copyright © 2000 John Wiley & Sons, Ltd.