Premium
Inheritance of suppressors of the drug sensitivity of a NSR1 deleted yeast strain
Author(s) -
Zabetakis Dan
Publication year - 2000
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/1097-0061(20000915)16:12<1147::aid-yea610>3.0.co;2-m
Subject(s) - biology , paromomycin , genetics , plasmid , ribosome , ribosomal rna , gene , mutant , mendelian inheritance , microbiology and biotechnology , rna , antibiotics , aminoglycoside
The NSR1 gene product is involved in ribosomal RNA production and ribosome assembly in Saccharomyces cerevisiae . Yeast strains carrying a deletion of the NSR1 gene have a defect in rRNA processing, an aberrant ribosome profile and are sensitive to the drug paromomycin. This paper reports the isolation and characterization of spontaneous suppressors of the paromomycin sensitivity. Such suppressors could be isolated at very high frequency and do not exhibit straightforward single‐gene inheritance patterns. The suppressors are not influenced by non‐Mendelian factors such as Ψ or ρ. Through a replacement of chromosomal rDNA with a plasmid rDNA system, I show that suppression of paromomycin sensitivity is mediated by rDNA. Swapping wild‐type plasmid rDNA for chromosomal rDNA can reverse the suppression, but the effect does not appear to be due to amplification of rDNA or amplification of a pre‐existing mutant rDNA copy. Copyright © 2000 John Wiley & Sons, Ltd.