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Cloning and sequence analysis of the Candida boidinii ADE2 gene
Author(s) -
Komeda Toshihiro,
Suda Hisako,
Sakai Yasuyoshi,
Kato Nobuo,
Tamai Yukio
Publication year - 2000
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/1097-0061(200007)16:10<953::aid-yea590>3.0.co;2-d
Subject(s) - biology , gene , homology (biology) , nucleic acid sequence , open reading frame , sequence analysis , cloning (programming) , genetics , saccharomyces cerevisiae , peptide sequence , yeast , microbiology and biotechnology , molecular cloning , biochemistry , computer science , programming language
Candida boidinii ADE2 gene (phosphoribosyl‐5‐aminoimidazole carboxylase; AIRC, EC 4. 1. 1. 21) has been cloned by homology to the Saccharomyces cerevisiae ADE2 gene. The cloned C. boidinii ADE2 gene complemented the ade2 mutation of S. cerevisiae . Sequence analysis showed a single open reading frame of 1719 nucleotides coding for a polypeptide of 573 residues. Comparison of the deduced amino acid sequence with those of AIRC enzymes from other yeasts showed marked homology among yeast AIRCs. The sequence has already been submitted to DDBJ/EBML/GenBank under Accession No. AB034950. Copyright © 2000 John Wiley & Sons, Ltd.