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Yeast two‐hybrid systems and protein interaction mapping projects for yeast and worm
Author(s) -
Walhout Albertha J. M.,
Boulton Simon J.,
Vidal Marc
Publication year - 2000
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/1097-0061(20000630)17:2<88::aid-yea20>3.0.co;2-y
Subject(s) - biology , budding yeast , caenorhabditis elegans , yeast , saccharomyces cerevisiae , computational biology , proteome , genome , protein–protein interaction , model organism , identification (biology) , gene , genetics , functional genomics , genomics , ecology
The availability of complete genome sequences necessitates the development of standardized functional assays to analyse the tens of thousands of predicted gene products in high‐throughput experimental settings. Such approaches are collectively referred to as ‘functional genomics’. One approach to investigate the properties of a proteome of interest is by systematic analysis of protein–protein interactions. So far, the yeast two‐hybrid system is the most commonly used method for large‐scale, high‐throughput identification of potential protein–protein interactions. Here, we discuss several technical features of variants of the two‐hybrid systems in light of data recently obtained from different protein interaction mapping projects for the budding yeast Saccharomyces cerevisiae and the nematode Caenorhabditis elegans . Copyright © 2000 John Wiley & Sons, Ltd.