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Rapid and reliable protein extraction from yeast
Author(s) -
Kushnirov Vitaly V.
Publication year - 2000
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/1097-0061(20000630)16:9<857::aid-yea561>3.0.co;2-b
Subject(s) - yeast , biology , saccharomyces cerevisiae , extraction (chemistry) , electrophoresis , agar , protein purification , biochemistry , strain (injury) , agar plate , chromatography , genetics , chemistry , bacteria , anatomy
The methods currently used for protein extraction from yeast are either laborious or insufficiently reliable. Here I report a method for protein extraction for electrophoretic analysis that is both easy and reliable. In this method, yeast cells are subjected to mild alkali treatment and then boiled in a standard electrophoresis loading buffer. The method was tested for different strains of Saccharomyces cerevisiae and for yeast Hansenula polymorpha DL‐1. It yields virtually complete extraction independently of the strain, growth conditions and protein molecular weight and allows working with small amounts of yeast cells grown on agar plates. Copyright © 2000 John Wiley & Sons, Ltd.