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Rat model of experimentally induced abacterial prostatitis
Author(s) -
Lang Michael D.,
Nickel J. Curtis,
Olson Merle E.,
Howard Sean R.,
Ceri Howard
Publication year - 2000
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/1097-0045(20001101)45:3<201::aid-pros1>3.0.co;2-q
Subject(s) - inflammation , prostatitis , tumor necrosis factor alpha , cytokine , prostate , interleukin , pathology , medicine , endocrinology , immunology , cancer
BACKGROUND An experimental model in rats was developed to investigate the significance of mucosal integrity in abacterial prostatitis. METHODS Ethanol was instilled into the ventral prostates of male rats to reduce mucosal integrity; dinitrobenzenesulfonic acid (DNBS) was added as an irritant to induce inflammation. Controls received no treatment, ethanol only, DNBS only, or a suspension of bacteria. After various time points, rats were sacrificed, and their prostates were assayed for gross morphology, histological appearance, and cytokine levels. RESULTS Prostates subjected to ethanol plus DNBS showed significant inflammation, most notably after 12, 24, and 48 hr. Inflammation judged by gross and histological observations and interleukin‐1β levels correlated well at these times. Rats given only ethanol , DNBS, or no treatment, acting as negative controls, displayed little or no inflammation; rats given a bacterial suspension, acting as positive controls, showed inflammation consistent with past studies. Cytokine assays revealed raised interleukin‐1β levels in this model, while tumor necrosis factor‐α remained at a basal level. CONCLUSIONS The loss of an intact mucosal surface in the prostate resulted in inflammation caused by an irritant. Interleukin‐1β appears to play a role in this inflammation, while tumor necrosis factor‐α does not. Prostate 45:201–206, 2000. © 2000 Wiley‐Liss, Inc.