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Analysis of type T1 and T2 cytokines in patients with prostate cancer
Author(s) -
Filella Xavier,
Alcover Juan,
Zarco Maria Angeles,
Beardo Pastora,
Molina Rafael,
Ballesta Antonio Manuel
Publication year - 2000
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/1097-0045(20000901)44:4<271::aid-pros2>3.0.co;2-g
Subject(s) - ionomycin , medicine , prostate , prostate cancer , cancer , endocrinology , basal (medicine) , proinflammatory cytokine , stimulation , immunology , inflammation , insulin
Abstract BACKGROUND It has been proposed that a dysregulation in the balance between type T1 (IL‐2, IFN‐γ) and type T2 (IL‐4, IL‐10) cytokines may be implicated in the development of cancer. METHODS We determined the expression of IL‐2, IL‐4, IL‐10, and IFN‐γ in CD4 and CD8 lymphocytes by flow cytometry in 12 patients with prostate cancer and in 7 healthy subjects. In addition to the basal expression of these cytokines, their expression was also determined, following stimulation of lymphocytes with PMA (phorbol 12‐mystirate 13 acetate) and ionomycin. RESULTS The basal expression of cytokines was scarce, while following stimulation this increased markedly. On the other hand, there was a dysregulation in the balance between T1 and T2 lymphocytes in patients with prostate cancer. To this effect, in relation to healthy subjects, we observed an increase in IL‐10 expression and a decrease in IL‐2 expression. CONCLUSIONS The disequilibrium observed in the balance between type T1 and type T2 cytokines may be implicated in the evolution of neoplastic disease. Prostate 44:271–274, 2000. © 2000 Wiley‐Liss, Inc.