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Cell death in the choroid plexus following transient forebrain global ischemia in the rat
Author(s) -
FerrandDrake Michel
Publication year - 2000
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/1097-0029(20010101)52:1<130::aid-jemt14>3.0.co;2-6
Subject(s) - choroid plexus , programmed cell death , forebrain , tunel assay , population , ischemia , hippocampus , biology , neuroscience , microbiology and biotechnology , pathology , medicine , apoptosis , central nervous system , immunohistochemistry , biochemistry , environmental health
Following a complete disruption of blood flow to the brain, cerebral ischemia, a specific neuronal population, namely the CA1 pyramidal neurons in the hippocampus, will die a delayed type of cell death. This is often referred to as “delayed neuronal death” (DND). It is not known why it takes around 48 hours for these cells to die. It is very often speculated that events, intrinsic to the CA1 neurons, regulate their demise, whereas it is less often considered that extrinsic mechanisms also could play an important role for the development of DND. We discovered that in addition to the CA1 pyramidal neurons, cells in the choroid plexus were TUNEL (terminaldeoxynucleotidyl‐mediated biotin‐dUTP nick‐end labeling)‐positive following transient forebrain global ischemia. The time course and the number of TUNEL‐positive cells were determined. A dramatic increase in the number of TUNEL‐positive cells in the choroid plexus was seen at 18, 24, and at 36 hours of recovery, but not at 48 hours of recovery following 15 minutes of transient forebrain global ischemia. No TUNEL‐positive cells were seen at 24 hours of recovery in the CA1 region. The cell death in the choroid plexus thus preceded the occurrence of cell death in the CA1 region. Massive cell death in the choroid plexus will inevitably lead to a leaky blood‐CSF barrier, which in turn will allow substances to enter the ventricular system and from there reach the brain parenchyma. We, therefore, conclude that choroid plexus cell death may adversely affect the outcome of CA1 pyramidal neurons following transient forebrain global ischemia, through, e.g., a disruption of the blood‐cerebro spinal fluid barrier. Alternatively, the choroid plexus may produce factors, which can affect the outcome of neurons. Microsc. Res. Tech. 52:130–136, 2001. © 2001 Wiley‐Liss, Inc.