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The beauty of the yeast: Live cell microscopy at the limits of optical resolution
Author(s) -
Kohlwein Sepp D.
Publication year - 2000
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/1097-0029(20001215)51:6<511::aid-jemt3>3.0.co;2-y
Subject(s) - yeast , microscopy , saccharomyces cerevisiae , confocal microscopy , staining , fluorescence microscope , confocal , microscope , resolution (logic) , biology , microbiology and biotechnology , deconvolution , biophysics , fluorescence , nanotechnology , chemistry , optics , materials science , biochemistry , computer science , artificial intelligence , physics , genetics
The yeast Saccharomyces cerevisiae is a very powerful system for cell biological research. Recent advances in electronic light microscopy together with the application of green fluorescent protein and other in vivo staining techniques have allowed novel and exciting insights into structural organization and dynamics of cells as small as yeast. Methods for staining yeast for microscopic inspection and for introducing tags for localization studies of proteins in living or fixed cells are summarized. Electronic light microscopy, video/deconvolution methods, and confocal laser scanning microscopy as novel tools for structural analyses, and their practical applications in yeast, are discussed. Microsc. Res. Tech. 51:511–529, 2000. © 2000 Wiley‐Liss, Inc.