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Detection of membrane‐bound tumor necrosis factor (TNF): An analysis of TNF‐specific reagents
Author(s) -
Gerspach Jeannette,
Götz Alex,
Zimmermann Gudrun,
Kolle Carmen,
Böttinger Heiner,
Grell Matthias
Publication year - 2000
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/1097-0029(20000801)50:3<243::aid-jemt8>3.0.co;2-b
Subject(s) - tumor necrosis factor alpha , transmembrane protein , cytokine , receptor , transmembrane domain , cytokine receptor , antibody , microbiology and biotechnology , biology , fusion protein , recombinant dna , chemistry , immunology , biochemistry , gene
Tumor necrosis factor (TNF) exists in two bioactive forms, the membrane integrated form and the proteolytically derived soluble cytokine. Both forms of TNF are involved in a variety of different physiological and pathophysiological situations. Here we analyzed different human and mouse TNF‐specific reagents for their ability to determine the expression of membrane‐expressed TNF. The data prove some antibodies to be very useful for the analysis of transmembrane TNF expression because these antibodies distinguish between the transmembrane form of TNF and soluble TNF bound to cellular TNF receptors. In addition, we found that recombinant human TNF receptor fusion proteins are advantageous tools to analyze both human and mouse transmembrane TNF expression. Microsc. Res. Tech. 50:243–250, 2000. © 2000 Wiley‐Liss, Inc.