Premium
Electrophoretic identification of hard white spring wheats grown at different locations in Pakistan in different years
Author(s) -
Anjum Faqir M,
Lookhart G L,
Walker C E
Publication year - 2000
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/1097-0010(200006)80:8<1155::aid-jsfa587>3.0.co;2-j
Subject(s) - spring (device) , cultivar , gliadin , electrophoresis , botany , chemistry , biology , chromatography , food science , physics , gluten , thermodynamics
Pakistani wheats were identified via acid polyacrylamide gel electrophoresis (A‐PAGE) of gliadin proteins. Gliadin electrophoretograms of wheat cultivars were qualitatively independent of growth locations and years. The Pakistani wheat Faisalabad 83 and the American wheat KS84HW196 contained an intense gliadin band in the region of the relative mobility 50 band of Marquis. That band was readily distinguishable and was followed by distinct intense double bands in the same position as those of Marquis, and can be used as a reference for mobility assignments in wheat cultivar identification work. Minor differences in band intensities across locations and years were noticed. Identification tables based on band mobilities relative to Marquis and relative intensities were established. © 2000 Society of Chemical Industry