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Unlocking the archive – gene expression in paraffin‐embedded tissue
Author(s) -
Lewis F.,
Maughan N. J.,
Smith V.,
Hillan K.,
Quirke P.
Publication year - 2001
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/1096-9896(200109)195:1<66::aid-path921>3.0.co;2-f
Subject(s) - polymerase chain reaction , dna microarray , in situ hybridization , reverse transcriptase , biology , gene expression , gene , reverse transcription polymerase chain reaction , computational biology , tissue microarray , real time polymerase chain reaction , gene expression profiling , microbiology and biotechnology , pathology , immunohistochemistry , genetics , medicine , immunology
The histopathology archive represents a vast, well‐characterized source of specimens covering virtually every disease and is available for molecular biological investigation. The archive has in recent years become widely used for molecular genetic analysis and DNA can be routinely extracted from formalin‐fixed, paraffin‐embedded tissue. More recently, archival specimens have become a source of material for extensive analysis of mRNA expression utilizing DNA microarrays, real‐time quantitative reverse transcriptase polymerase chain reaction (PCR), and insitu hybridization and amplification techniques. These techniques will enable a greater understanding of the changes that occur in gene function during every stage of the development of disease and will lead to better diagnosis, better evaluation of prognosis, and better treatment through targeted therapeutic regimes. Copyright © 2001 John Wiley & Sons, Ltd.