Premium
Methods for imaging the structure and function of living tissues and cells: 2. Fluorescence lifetime imaging
Author(s) -
Tadrous Paul J.
Publication year - 2000
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/1096-9896(200007)191:3<229::aid-path623>3.0.co;2-b
Subject(s) - fluorescence lifetime imaging microscopy , fluorescence , function (biology) , biology , microbiology and biotechnology , physics , optics
This second article in the series shows how fluorescence lifetime imaging allows natural biochemical and physiological properties of tissues to act as contrast agents and so provide a basis for distinguishing normal and diseased tissue components. When combined with methods for imaging through non‐transparent tissues and tomographic reconstruction it shows promise as a new optical biopsy technique. In addition to this, specially designed vital fluorescent probes of specific biochemical, secondary messenger and receptor activity in living cells may be imaged using FLIM. This is the youngest of the techniques covered in these review articles on imaging, the first FLIM images of cells having been produced in 1994. Copyright © 2000 John Wiley & Sons, Ltd.