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Endothelial capillaries chemotactically attract tumour cells
Author(s) -
von Bülow Cornelie,
Hayen Wiebke,
Hartmann Anke,
MuellerKlieser Wolfgang,
Allolio Bruno,
Nehls Volker
Publication year - 2001
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/1096-9896(2000)9999:9999<::aid-path810>3.0.co;2-1
Subject(s) - chemotaxis , umbilical vein , microcarrier , microbiology and biotechnology , paracrine signalling , endothelial stem cell , angiogenesis , endothelium , cell migration , biology , cell culture , chemotaxis assay , metastasis , cancer research , immunology , in vitro , cancer , receptor , biochemistry , genetics , endocrinology
Directional migration of capillaries towards tumour implants is generally assumed to be regulated by chemotaxis. Preliminary evidence has also been presented for the existence of a reverse chemotactic signalling pathway, with capillaries attracting tumour cells via paracrine factors. By using a variety of endothelial cell types and tumour cell lines, this study has systematically investigated chemotaxis between endothelial cells and tumour cells in two‐ and three‐dimensional systems. Checkerboard analysis revealed faint attraction of human umbilical vein endothelial cells (HUVECs), but not porcine aortic endothelial cells (PAECs), by tumour cells. In reverse, both PAECs and HUVECs potently induced chemotactic migration of tumour cells. Using a microcarrier‐based fibrin gel assay, directional migration of endothelial cells towards tumour cells was not observed. In reverse, tumour cells were strongly attracted by endothelial cells. Identification of endothelium‐derived chemotactic molecules may provide a valuable approach for the treatment of tumour metastasis. Copyright © 2000 John Wiley & Sons, Ltd.