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Validation of a model of colon cancer progression
Author(s) -
Hewitt Robert E.,
McMarlin Andrew,
Kleiner David,
Wersto Robert,
Martin Patrick,
Tsoskas Maria,
Stamp Gordon W. H.,
StetlerStevenson William G.
Publication year - 2000
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/1096-9896(2000)9999:9999<::aid-path775>3.0.co;2-k
Subject(s) - cell culture , cancer research , colorectal cancer , metastasis , biology , in vitro , phenotype , fibroblast , cancer , cell , pathology , immunology , medicine , genetics , gene
A unique feature of SW480 and SW620 colon carcinoma cell lines is that they are derived from primary and secondary tumours resected from a single patient. As such, they may represent a valuable resource for examining genetic changes late in colon cancer progression. In order to verify this, both cell lines have been characterized to determine whether phenotypic differences have been retained despite long‐term cell culture in vitro . The primary tumour‐derived SW480 cells have an epithelioid morphology in vitro , while metastasis‐derived SW620 cells have a fibroblast‐like appearance. Xenografts of SW480 cells form gland‐like structures in vivo , while SW620 xenografts form solid sheets of tumour cells. SW620 cells have a higher BrdU labelling index than SW480 cells, and are more highly tumourigenic and metastatic. Furthermore, SW620 cells show less susceptibility to apoptosis induction by TNF α and anti‐Fas monoclonal. Findings from these investigations therefore indicate that SW480 and SW620 cell lines do show appropriate phenotypic differences and represent an interesting model for studying the genetic events in the late stages of colon cancer progression. Copyright © 2000 John Wiley & Sons, Ltd.