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Determination of ondansetron and its hydroxy metabolites in human serum using solid‐phase extraction and liquid chromatography/positive ion electrospray tandem mass spectrometry
Author(s) -
Xu Xiaohui,
Bartlett Michael G.,
Stewart James T.
Publication year - 2000
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/1096-9888(200011)35:11<1329::aid-jms67>3.0.co;2-g
Subject(s) - chemistry , chromatography , solid phase extraction , electrospray ionization , detection limit , ammonium acetate , tandem mass spectrometry , elution , mass spectrometry , electrospray , liquid chromatography–mass spectrometry , selected reaction monitoring , extraction (chemistry) , high performance liquid chromatography
Ondansetron and its hydroxylated metabolites were determined in human serum using solid‐phase extraction (SPE) and liquid chromatography/positive ion electrospray tandem mass spectrometry. Pyrimethamine was used as the internal standard. The analytes were eluted from the SPE cartridge using 2 × 1 ml of methanol containing 0.5% triethylamine, evaporated under vacuum and the residue was reconstituted in the mobile phase. The liquid chromatographic separation was achieved on a silica column using a mobile phase of aqueous 20 m M ammonium acetate (pH 4.7)–acetonitrile (85 : 15, v/v) at a flow‐rate of 0.4 ml min −1 . The method was linear over the range 1–500 ng ml −1 for ondansetron and each of the metabolites in human serum. The intra‐day accuracy was better than 9.1% and the precision was <10.3%; the inter‐day accuracy was better than 9.5% and the precision was <12.6%. The limit of detection was 250 pg ml −1 based on a signal‐to‐noise ratio of 3. The absolute recovery from serum for all analytes was >90%. Copyright © 2000 John Wiley & Sons, Ltd.