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Identification and characterization of Podophyllum emodi by API‐LC/MS/MS
Author(s) -
Wong SiuKay,
Tsui ShuKi,
Kwan SikYiu,
Su XiaoLi,
Lin RuiChao
Publication year - 2000
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/1096-9888(200011)35:11<1246::aid-jms55>3.0.co;2-l
Subject(s) - lignan , chemistry , podophyllum , gentiana , medicinal herbs , traditional medicine , tandem mass spectrometry , herb , echinacea (animal) , chromatography , mass spectrometry , podophyllotoxin , stereochemistry , medicine
An API‐LC/MS/MS method was developed for the identification of the medicinal herb Podophyllum emodi based on the profile of its aryltetrahydronapthalene and related lignan marker compounds. This was done by matching the structural information from the tandem mass spectrometric data with those lignan marker compounds already reported for the herb. The method could be employed in the absence of reference standards for the markers and was particularly useful in view of the scarcity of supply of these chemical standards. It has been used successfully to differentiate Podophyllum emodi from two commonly used medicinal herbs of a different genus but having similar appearance, Radix clematidis and Radix gentiana , as well as a closely related herb, Podophyllum peltatum . Copyright © 2000 John Wiley & Sons, Ltd.