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Selenium speciation in animal tissues after enzymatic digestion by high‐performance liquid chromatography coupled to inductively coupled plasma mass spectrometry
Author(s) -
Quijano M. Angeles,
Moreno Patricia,
Gutiérrez Ana Maria,
PérezConde M. Concepción,
Cámara Carmen
Publication year - 2000
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/1096-9888(200007)35:7<878::aid-jms12>3.0.co;2-2
Subject(s) - chemistry , selenium , chromatography , selenate , inductively coupled plasma mass spectrometry , enzymatic hydrolysis , mass spectrometry , microwave digestion , sample preparation , detection limit , hydrolysis , biochemistry , organic chemistry
A procedure is described for the enzymatic digestion of tuna and mussel samples that allows the determination of selenium species by high‐performance liquid chromatography in conjunction with inductively coupled plasma mass spectrometry. The species were extracted by two‐step enzymatic hydrolysis with a non‐specific protease (subtilisin). The selenium species were separated on a Spherisorb 5 ODS/AMINO column using two different chromatographic conditions, namely phosphate buffers at pH 2.8 and pH 6.0 as mobile phases. The method determines organic (trimethylselenonium, selenocystine, selenomethionine and selenoethionine) and inorganic selenium species (selenite and selenate), but only organic selenium species were found in the samples. The sum of identified selenium species in the sample was about 30% of the total selenium present in the enzymatic extract despite the fact that recoveries of total hydrolysed selenium were 93–102%. Trimethylselenonium ion and selenomethionine were found in both tuna and mussel samples and an unknown selenium species was also found in tuna samples. Copyright © 2000 John Wiley & Sons, Ltd.