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Mass spectrometric analysis of hydroxyproline glycans
Author(s) -
Kilz S.,
Waffenschmidt S.,
Budzikiewicz H.
Publication year - 2000
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/1096-9888(200006)35:6<689::aid-jms995>3.0.co;2-t
Subject(s) - chemistry , hydroxyproline , glycan , chromatography , dissociation (chemistry) , electrospray ionization , collision induced dissociation , hydrolysis , mass spectrometry , ion , sugar , electrospray , yield (engineering) , fragmentation (computing) , quadrupole ion trap , alkaline hydrolysis , glycoprotein , ion trap , tandem mass spectrometry , organic chemistry , biochemistry , materials science , computer science , metallurgy , operating system
Mass spectrometric techniques are presented which allow one to analyze the sugar part bound to hydroxyproline in hydroxyproline‐rich glycoproteins. The hydroxyproline (Hyp) glycans obtained by alkaline hydrolysis give abundant [M + Na] + ions by electrospray ionization which after collision‐induced dissociation (CID) yield inter alia [Hyp − H + Na] + . In mixtures a parent ion scan of this species will indicate the various molecular species which can then be analyzed by MS n after CID in an ion trap, where successive losses of the sugar units are observed. Methylation techniques allow one to distinguish between linear and branched isomeric structures. Copyright © 2000 John Wiley & Sons, Ltd.