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Differential distribution of nerve terminals immunoreactive for substance P and cholecystokinin in the sympathetic preganglionic cell column of the filefish Stephanolepis cirrhifer
Author(s) -
Funakoshi Kengo,
Kadota Tetsuo,
Atobe Yoshitoshi,
Nakano Masato,
Goris Richard C.,
Kishida Reiji
Publication year - 2000
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/1096-9861(20001204)428:1<174::aid-cne12>3.0.co;2-u
Subject(s) - substance p , biology , cholecystokinin , anatomy , neuropeptide , dorsal column nuclei , spinal cord , neuroscience , endocrinology , receptor , biochemistry
Immunoreactivity for substance P and cholecystokinin‐8 was examined in the nerve fibers in the central autonomic nucleus, a cell column for sympathetic preganglionic neurons, in the filefish Stephanolepis cirrhifer . Substance P‐immunoreactive fibers were distributed throughout the entire rostrocaudal extent, but were more abundant in the caudal part of the column, where substance P‐immunoreactive varicosities sometimes made contacts with the sympathetic preganglionic neurons. Cholecystokinin‐8‐immunoreactive fibers were found almost entirely in the rostral part of the column, where a dense network of varicosities was in close apposition to a considerable number of the sympathetic preganglionic neurons. Double labeling immunohistochemistry showed that substance P fibers and cholecystokin‐8 fibers were entirely different, and distinct from serotonin‐immunoreactive fibers. By using immunoelectron microscopy, synaptic specialization was sometimes observed between the dendrites of preganglionic neurons and varicosities immunoreactive for substance P and cholecystokinin‐8. Substance P‐ and cholecystokinin‐8 fibers were seen from the descending trigeminal tract, through the dorsolateral funiculus and the ventral portion of the dorsal horn, to the central autonomic nucleus. After colchicine treatment, substance P‐immunoreactive perikarya were found in the cranial and spinal sensory ganglia. These results suggest that the sympathetic preganglionic neurons of the filefish receive innervation by substance P fibers and cholecystokinin fibers, and that the former might be of primary sensory origin. Topographical distribution of cholecystokinin‐8‐immunoreactive terminals in the central autonomic nucleus along the rostrocaudal extent might underlie the differential regulation of sympathetic activity via a distinct population of sympathetic preganglionic neurons. J. Comp. Neurol. 428:174–189, 2000. © 2000 Wiley‐Liss, Inc.