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Bactericidal effect of pulsed 1,064 nm Nd:YAG laser light on Staphylococcus epidermidis is of photothermal origin: An in vitro study
Author(s) -
Grönqvist Anders,
Wiström Johan,
Axner Ove,
Monsen Tor Johan
Publication year - 2000
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/1096-9101(2000)27:4<336::aid-lsm6>3.0.co;2-h
Subject(s) - photothermal therapy , staphylococcus epidermidis , laser , radiation , irradiation , optics , materials science , photothermal effect , chemistry , bacteria , staphylococcus aureus , nanotechnology , biology , physics , nuclear physics , genetics
Background and Objective The aim was to investigate the bactericidal effect of the 1,064 nm Nd:YAG laser on Staphylococcus epidermidis . Material and Methods S. epidermidis was inoculated on agar plates and then exposed to pulsed laser light in three different modes: with an uninterrupted train of pulses, or with two different repeated cycles of fractionated trains of pulses. The agar temperature was measured directly after uninterrupted radiation. Results A bacterial growth inhibition area of 0.3 cm 2 and maximum temperature of approximately 80°C was observed after uninterrupted radiation at 2,000 J cm −2 . The corresponding figures after an exposure of 5,000 J cm −2 were 0.9 cm 2 and 100°C, respectively. No bacterial inhibition was observed after exposure to repeated cycles of 20 seconds of radiation followed by 60 seconds of rest. Conclusion The antimicrobial effect of the 1,064 nm Nd:YAG laser light is caused by a photothermal rather than a photochemical effect. Lasers Surg. Med. 27:336–340, 2000. © 2000 Wiley‐Liss, Inc.