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New technique for producing hybridoma by using laser radiation
Author(s) -
Ohkohchi Nobuhiro,
Itagaki Hideo,
Doi Hideyuki,
Taguchi Yoshio,
Satomi Susumu,
Satoh Syunichi
Publication year - 2000
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/1096-9101(2000)27:3<262::aid-lsm8>3.0.co;2-q
Subject(s) - laser , cell fusion , fusion , irradiation , materials science , microscopy , cell , microscope , lymphocyte , optics , chemistry , biology , immunology , biochemistry , physics , linguistics , philosophy , nuclear physics
Background and Objective With conventional methods, cell fusion rate is extremely low, and fusion of two specific cells is not possible. We developed a new method for inducing cell fusion under the microscope by using a microprocessing device by laser. Study Design/Materials and Methods Under a microscope, the target cells were irradiated with laser beams for trapping. Then, the trapped cells were transferred and placed in contact with the corresponding cells, which were also fixed by laser beam. The pulse laser beams are focused on the contact surface to cut small perforations for mutual communication of the cytoplasms. Results The fusion rate of mouse myeloma cells was 38%. The rate of hybridoma production of myeloma cell and lymphocyte was 2%. We confirmed the proliferation of the newly formed hybridoma in HAT medium and the production of immunoglobulin G. Conclusion This new cell fusion method is characterized by production of hybridomas of target cells, lower cell toxicity, and a high rate of hybrid production. Lasers Surg. Med. 27:262–268, 2000. © 2000 Wiley‐Liss, Inc.