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Laser irradiative tissue probed in situ by collagen 380‐nm fluorescence imaging
Author(s) -
Tang Jing,
Zeng Fanan,
Savage Howard,
Ho Peng Pei,
Alfano R.R.
Publication year - 2000
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/1096-9101(2000)27:2<158::aid-lsm7>3.0.co;2-i
Subject(s) - fluorescence , in situ , laser , histology , stain , irradiation , materials science , laser induced fluorescence , fluorescence lifetime imaging microscopy , biomedical engineering , chemistry , pathology , optics , staining , medicine , physics , organic chemistry , nuclear physics
Background and Objective There is no ideal method to detect molecular changes in situ of laser‐irradiated tissue without removing a section of tissue for histologic examination. A new method is reported to evaluate laser‐induced thermal tissue damage in situ by using 380‐nm native fluorescence imaging under 340‐nm excitation. Study Design/Materials and Methods Native fluorescence imaging was performed on laser irradiated bovine tendon tissue and compared with light illuminated photography and histology with picrosirius red F3BA stain. Results and Conclusion The results indicate that a decrease in collagen fluorescence at 380 nm was observed in laser‐induced thermally damaged tissues. The injured region of tissue defined by its fluorescence image coincided with an area defined by photography and histology. Lasers Surg. Med. 27:158–164, 2000. © 2000 Wiley‐Liss, Inc.