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Bacteria identification of otitis media with fluorescence spectroscopy
Author(s) -
Sorrell Matthew J.,
Tribble Jerri,
Reinisch Lou,
Werkhaven Jay A.,
Ossoff Robert H.
Publication year - 1994
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/1096-9101(1994)14:2<155::aid-lsm1900140208>3.0.co;2-l
Subject(s) - fluorescence , fluorescence spectroscopy , otitis , absorption (acoustics) , bacteria , chemistry , analytical chemistry (journal) , microbiology and biotechnology , biology , chromatography , medicine , optics , physics , surgery , genetics
We have investigated the fluorescence profiles of four common pathogens: S. pneumoniae, S. aureus, M. catarrhalis , and H. influenzae . The steady‐state auto fluorescence spectra of bacteria are measured as a function of the incident light from 200 to 700 nm. The spectra for each bacterium are combined into a fluorescence profile or fluorescence finger print. Each bacterium produces a unique in vitro fluorescence profile when measured in a saline suspension. The profiles are reproducible. Suspensions of a bacterial strain, where the identification is not known, can be correctly matched to a small library of previously measured fluorescence profiles using a linear least‐squares fitting algorithm. In addition, we have measured the fluorescence and absorption spectrum of the tympanic membrane removed from a chinchilla. The optical properties of the tympanic membrane and the least‐squares identification process form precept for a non‐invasive, fluorescence based bacterial diagnosis technique to be used in otitis media. © 1994 Wiley‐Liss, Inc.